PARENTAL GENOME DIFFERENTIATION BY GENOMIC IN SITU HYBRIDIZATION (GISH) IN INTERGENERIC HYBRID OF SACCHARUM X ZEA
نویسندگان
چکیده
منابع مشابه
Identifying parental chromosomes and genomic rearrangements in animal hybrid complexes of species with small genome size using Genomic In Situ Hybridization (GISH)
Genomic In Situ Hybridization (GISH) is a powerful tool to identify and to quantify genomic constituents in allopolyploids, and is mainly based on hybridization of highly and moderate repetitive sequences. In animals, as opposed to plants, GISH has not been widely used in part because there are technical problems in obtaining informative results. Using the allopolyploid Squalius alburnoides Ste...
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to remove unfavorable traits of new cereal tritipyrum (2n=6x=42, aabbebeb), various crosses between primary tritipyrum lines (female) with iranian bread wheat varieties (2n=6x=42, aabbdd) was performed and a f2 selfing population consisted of 1810 possible secondary tritipyrum seeds (2n=6x=42, aabbd(0-14) eb(0-14)) were produced. the chromosome constitution and homology index of 11 plants of th...
متن کاملEstablishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora.
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni...
متن کامل[Genomic in situ hybridization in intergeneric hybrids between Raphanus sativus and Brassica oleracea].
Genomic in situ hybridization (GISH) was applied to study the meiosis of F1 plants from intergeneric hybrids between radish (Raphanus sativus, 2n=18, RR) and cabbage (Brassica oleracea, 2n=18, CC). The result showed that its somatic cells had the expected chromosomes, RC, 2n=18; but the pollen mother cells (PMCs) were different. There were three main kinds of PMCs. The first one was RC (2n=18),...
متن کاملEvaluation of Discontinues Puresperm Gradient in Separation of X And Y Bearing Human Spermatozoa by Fluorescent in Situ Hybridization (FISH)
Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...
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ژورنال
عنوان ژورنال: Journal of Sugarcane Research
سال: 2019
ISSN: 2582-4767,2249-927X
DOI: 10.37580/jsr.2019.2.9.130-137